下表中列出了几种限制酶识别序列及其切割位点,图1、图2中箭头表示相关限制酶的酶切

题型:读图填空题

问题:

下表中列出了几种限制酶识别序列及其切割位点,图1、图2中箭头表示相关限制酶的酶切位点。请回答下列问题:

(1)一个图1所示的质粒分子经SmaⅠ切割前后,分别含有______个游离的磷酸基团。

(2)若对图中质粒进行改造,插入的SmaⅠ酶切位点越多,质粒的热稳定性越_______________。

(3)用图中的质粒和外源DNA构建重组质粒,不能使用SmaⅠ切割,原因是_________________。

(4)与只使用EcoRⅠ相比较,使用BamHⅠ和HindⅢ两种限制酶同时处理质粒、外源DNA的优点在于可以防止_____________。

(5)为了获取重组质粒,将切割后的质粒与目的基因片段混合,并加入_______酶。

(6)重组质粒中抗生素抗性基因的作用是为了_________________。

(7)为了从cDNA文库中分离获取蔗糖转运蛋白基因,将重组质粒导入丧失吸收蔗糖能力的大肠杆菌突变体,然后在_____________的培养基中培养,以完成目的基因表达的初步检测。

考点:基因工程的基本操作程序DNA重组技术的基本工具
题型:读图填空题

有以下程序:
#include <stdio.h>
main()
char a='a', b;
printf("%c,", ++a);
printf(1%c\n", b=a++);

程序运行后的输出结果是( )。

A) b,b
B) b,c
C) a,b
D) a,c

题型:读图填空题

阅读理解。

     Welcome to Language upon Thames.  This brochure has been designed to give you an overview

of our Language School and the courses we offer. If you have some additional questions please do

not hesitate to contact us for more information.

    At Language upon Thames, we feel it is important to be flexible, in order that students can decide

what period of study suits them best.

          Small Group General English Courses

    These courses are aimed at students who wish to improve their speaking, listening, reading and

writing skills and are offered at the following levels.

    BEGINNERS

    ELEMENTARY (初级)

    PREINTERMEDIATE

    UPPERINTERMEDIATE

    Studying one of the above courses will enable you to use English more confidently and competently

on a daily basis.

    Try our free online test to see which level you are at-CLICK HERE.

                        Exam Courses

    These courses are aimed at students who wish to gain academic qualifications in English and are

offered at the following levels.

    University of Cambridge exams:

    ADVANCED 1-FCE (First certificate)

    ADVANCED 2-CAE (Advanced)

    ADVANCED 3-CPE (Proficiency)

    Studying one of the above courses will enable you to continue your education or enter university in

this country. (Students wishing to gain admission to a British university are normally required to have

the Cambridge Proficiency Certificate.)

           Speaking, Listening & Pronunciation

     This course builds on communicative confidence and competence and is aimed at students who

wish to develop the important skills of speaking and listening.

     Emphasis is also placed on pronunciation, with activities designed to meet the needs of students of

different nationalities, who need to focus on different areas.

     CLICK HERE to register for a General English course.

                      Other Languages

     At Language upon Thames we offer a wide range of cafeterias, restaurants, shops and bars. Most

importantly, we have foreign language classes of French, Japanese, German, Spanish and Italian during

the day, evening or on a onetoone basis.

1. What does this passage mainly talk about? 

A. English courses.

B. Ways to improve students' English.

C. The best way to improve your skills.

D. The importance of English.

2. If you attend Exam Courses, you will ________. 

A. use English as fluently as a native speaker

B. deal with everyday English well

C. have better pronunciation of English

D. be able to continue your education or enter university in this country

3. Judging from the description, we can tell that this passage must be taken from ________.  

A. a newspaper              

B. a magazine

C. the Internet              

D. a guidebook

4. The following statements are true EXCEPT ________. 

A. only Englishmen can attend the courses

B. the courses are designed for learners of different levels

C. one can learn French even in the evening

D. Language upon Thames is a school aimed to offer help with language learning

题型:读图填空题

资产计量最重要的基础是()

A、现金成本

B、历史成本

C、现行市价

D、可变现净值

题型:读图填空题

因抢救危急患者未能及时书写病历的,医务人员应在抢救结束后多少小时补写病历()

A.3小时

B.6小时

C.12小时

D.24小时

E.48小时

题型:读图填空题

根据本季度生产计划完成情况,对下季度生产应作如何调整()

A.增加甲产品计划数,减少乙产品的生产计划

B.乙产品的计划不变,增加丙产品和甲产品的计划

C.增加了产品计划,同时维持甲产品在原有水平,减少乙产品的生产计划

D.增加丁产品和丙产品的计划,乙产品维持原有水平,减少甲产品计划

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